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Ere fixed in 1.5 glutaraldehyde (Serva) diluted in 0.1M cacodylate buffer, pH 7.4 stored for 20 min at room temperature, post-fixed in 1 OsO4 for 60 min at 4 , dehydrated in ethanol, and finally embedded in Dow Epoxy Resin (DER 332, Unione Chimica Europea, Milano, Italy). For double immunogold labelling ultrathin sections, cut by an ultramicrotome (Ultracut UCT; Leica, Wien, Austria) were mounte

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    zzhuaqiang

    zzhuaqiang 35 days ago Permalink

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